Noise induced hearing loss is a significant problem in the world today and despite being widely researched the fundamental mechanisms are not clear. Metabolomics is an emerging technology, which is highly sensitive in detecting complex biochemical changes occurring in an organ under various conditions. This powerful technique is not yet properly utilised in the field of auditory neuroscience. By examining the chemical changes that take place in our inner ear following noise induced stress, we hope to better understand how excessive noise can cause hearing loss and how we may be able to prevent it in future therapies. The outcome of this study can help us identify potential biomarkers from the diagnosis perspective.